کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8987930 | 1555139 | 2005 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Effects of environmental stressors on lymphocyte proliferation in Florida manatees, Trichechus manatus latirostris
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کلمات کلیدی
MMLUSGSPbTxBrdU - بروموداکسی اوریدینbromodeoxyuridine - برومودسوویریدینanalysis of variance - تحلیل واریانسANOVA - تحلیل واریانس Analysis of variancefMRI - تصویرسازی تشدید مغناطیسی کارکردیLymphocyte proliferation - تکثیر لنفوسیتUnited States Geological Survey - سازمان زمین شناسی ایالات متحدهstimulation index - شاخص تحریکImmune function - عملکرد ایمنیManatee - مناته
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The health of many Florida manatees (Trichechus manatus latirostris) is adversely affected each year by exposure to cold weather or harmful algal blooms (red tide; Karenia brevis). Exposures can be sublethal, resulting in stressed animals that are rescued and taken to authorized facilities for rehabilitation, or lethal if exposures are prolonged or unusually severe. To investigate whether sublethal environmental exposures can impair immune function in manatees, rendering animals vulnerable to disease or death, mitogen-induced proliferation was assessed in lymphocytes from manatees exposed to cold temperatures (NÂ =Â 20) or red tide (NÂ =Â 19) in the wild, and compared to lymphocyte responses from healthy free-ranging manatees (NÂ =Â 32). All animals sampled for this study were adults. Lymphocytes were stimulated in vitro with either concanavalin A (ConA) or phytohemagglutinin (PHA) and proliferation was assessed after 96Â h using incorporation of the thymidine analog, bromodeoxyuridine (BrdU), into newly synthesized DNA. Proliferation of lymphocytes from manatees rescued from exposure to red tide or cold-stress was approximately one-third that of lymphocytes from healthy free-ranging manatees. To examine the direct effects of red tide toxins on lymphocyte function, mitogen-induced proliferation was assessed following co-culture of lymphocytes with K. brevis toxin extracts. Stimulation indices decreased with increasing toxin concentration, with a significant decrease in proliferation occurring in the presence of 400Â ng red tide toxins/ml. When lymphocytes from cold-stressed manatees were co-cultured with red tide toxin extracts, proliferative responses were reduced even further, suggesting multiple stressors may have synergistic effects on immune function in manatees.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Veterinary Immunology and Immunopathology - Volume 103, Issues 3â4, 10 February 2005, Pages 247-256
Journal: Veterinary Immunology and Immunopathology - Volume 103, Issues 3â4, 10 February 2005, Pages 247-256
نویسندگان
Cathy J. Walsh, Carl A. Luer, David R. Noyes,