کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8994289 | 1114277 | 2005 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Real-time detection and efficacy of antisense oligonucleotides delivered by PAMAM dendrimers in living cells
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کلمات کلیدی
موضوعات مرتبط
علوم پزشکی و سلامت
داروسازی، سم شناسی و علوم دارویی
اکتشاف دارویی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The aim of the present investigation was to study the behavior of PAMAM dendrimer-nucleic acid complexes in vitro and living cells. We demonstrated the rapid and sensitive detection of mRNA in living cells using molecular beacon pair, one with a donor and the other with a quenching fluorophore that hybridises to adjacent regions on the same mRNA target, resulting in fluorescence resonance energy transfer (FRET). The molecular beacon was composed of a 13-nt loop structure containing the antisense sequence that can hybridise with the AUG translational start site of the Friend env gene. It was shown that SuperFect may stimulate the antisense ON-RNA hybridisation. The secondary structure of antisense oligonucleotide was optimized. An antisense sequence-specific inhibition of 75% was obtained for one reporter gene with a stem-loop ODN containing four phosphorothioate groups, two at each end.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Drug Delivery Science and Technology - Volume 15, Issue 1, 2005, Pages 75-79
Journal: Journal of Drug Delivery Science and Technology - Volume 15, Issue 1, 2005, Pages 75-79
نویسندگان
A. Maksimenko, V. Helin, J.R. Bertrand, M. Gottikh, C. Malvy,