کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8998462 | 1115631 | 2005 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Protection of ischemic rat spinal cord white matter: Dual action of KB-R7943 on Na+/Ca2+ exchange and L-type Ca2+ channels
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کلمات کلیدی
EGTAOGDKB-R7943Na+–Ca2+ exchangeaxon - آکسون ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid - اتیلن گلیکول بیست (2-aminoethylether) -N، N، N '، N'-tetraacetic acidIschemia - ایسکمیTetraethylammonium - تترا اتیل آمونیومOxygen glucose deprivation - محرومیت گلوکز اکسیژنCompound Action Potential - پتانسیل اقدام مشترکTEA - چایCa2+ channels - کانال های Ca2 +CaP - کلاه لبه دار
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب رفتاری
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The effect of the Na+/Ca2+-exchange inhibitor KB-R7943 was investigated in spinal cord dorsal column ischemia in vitro. Oxygen/glucose deprivation at 37 °C for 1 h causes severe injury even in the absence of external Ca2+. KB-R7943 was very protective in the presence and absence of external Ca2+ implicating mechanisms in addition to extracellular Ca2+ influx through Na+/Ca2+-exchange, such as activation of ryanodine receptors by L-type Ca2+ channels. Indeed, blockade of L-type Ca2+ by nimodipine confers a certain degree of protection of dorsal column against ischemia; combined application of nimodipine and KB-R7943 was not additive suggesting that KB-R7943 may also act on Ca2+ channels. KB-R7943 reduced inward Ba2+ current with IC50 = 7 μM in tsA-201 cells expressing Cav1.2. Moreover, nifedipine and KB-R7943 both reduced depolarization-induced [Ca2+]i increases in forebrain neurons and effects were not additive. Nimodipine or KB-R7943 also reduced ischemic axoplasmic Ca2+ increase, which persisted in 0Ca2+/EGTA perfusate in dorsal column during ischemia. While KB-R7943 cannot be considered to be a specific Na+/Ca2+ exchange inhibitor, its profile makes it a very useful neuroprotectant in dorsal columns by: reducing Ca2+ import through reverse Na+/Ca2+ exchange; reducing influx through L-type Ca2+ channels, and indirectly inhibiting Ca2+ release from the ER through activation of ryanodine receptors.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuropharmacology - Volume 48, Issue 4, March 2005, Pages 566-575
Journal: Neuropharmacology - Volume 48, Issue 4, March 2005, Pages 566-575
نویسندگان
M. Ouardouz, G.W. Zamponi, W. Barr, L. Kiedrowski, P.K. Stys,