کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9030321 | 1130734 | 2005 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Genotoxicity evaluation of metronidazole using the piscine micronucleus test by acridine orange fluorescent staining
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موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بهداشت، سم شناسی و جهش زایی
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چکیده انگلیسی
In this study, genotoxic potential of metronidazole, an antibiotic-antiparasitic agent widely used both clinical and veterinary areas, was evaluated using the piscine micronucleus test. Specimens of Oreochromis niloticus (Pisces: Cichlidae) were exposed to different concentrations (5, 10, 15Â mg/L) of metronidazole and 4Â mg/L cyclophosphamide (as positive control) for 24, 48 and 72Â h. A fluorescent staining technique with acridine orange (AO) was performed to improve the sensitivity of erythrocyte micronucleus assay with fish. AO selectively stains newly formed immature erythrocytes (PCEs) therefore allows to obtain more sensitive results by detection of the micronucleated PCEs differed than mature erythrocytes (NCEs) by their RNA-containing cytoplasm. The ratio of PCE/NCE in peripheral blood was also evaluated to assess cytotoxicity. As a result, it was observed that the frequencies of micronucleated PCEs increased both dose and time dependently while PCE/NCE ratios decreased. Our results revealed that metronidazole has cytotoxic and genotoxic effects on fish. The use of acridine orange staining technique also seems useful in assessment of short-term genotoxic effects of chemicals, when fish are used as experimental animal.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Environmental Toxicology and Pharmacology - Volume 19, Issue 1, January 2005, Pages 107-111
Journal: Environmental Toxicology and Pharmacology - Volume 19, Issue 1, January 2005, Pages 107-111
نویسندگان
Tolga ÃavaÅ, Serap Ergene-Gözükara,