کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9102812 | 1152362 | 2005 | 14 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A novel model of drug hapten-induced hepatitis with increased mast cells in the BALB/c mouse
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کلمات کلیدی
dH2OTLR4NKTCYP2E1NaHCO3hpfpg/mlALTKOHTFAMSATNBSDIHHClPODAIH2,4,6-Trinitrobenzene sulfonic acid - 2،4،6-ترینیتروبنسل سولفونیک اسیدH&E - H & Edistilled water - آب مقطرAlanine aminotransferase - آلانین آمینوترانسفرازmouse serum albumin - آلبومین سرم موشhydrochloric acid - اسید هیدروکلریک یا اسید کلریدریک یا جوهر نمک AKP - حزب عدالت و توسعهsodium bicarbonate - سدیم بیکربناتnatural killer T cells - سلول های T قاتل طبیعیcytochrome P450 2E1 - سیتوکروم P450 2E1Cytokines - سیتوکین هاhigh-powered field - میدان مغناطیسیHematoxylin and Eosin - هماتوکسیلین و ائوزینAutoimmune hepatitis - هپاتیت اتوایمیونDrug-induced hepatitis - هپاتیت ناشی از مواد مخدرPotassium Hydroxide - پتاسیم هیدروکسیدToll-like receptor 4 - گیرنده تله مانند 4
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوشیمی بالینی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Clinical evidence suggests that idiosyncratic hepatitis following administration of halogenated volatile anesthetics is mediated by autoimmune responses. No murine model to study mechanisms of anesthetic-induced or any other form of drug-induced idiosyncratic hepatitis exists. Anesthetics are believed to trigger hepatitis by covalently linking a trifluoroacetyl (TFA) chloride hapten to hepatic proteins, forming haptenated self-proteins. To test this hypothesis, we developed a hapten-induced model of hepatitis by immunization with syngeneic S100 liver proteins covalently coupled to TFA (TFA-S100). We found that TFA-S100 induced hepatitis was more severe than disease induced by S100 plus adjuvants or by the adjuvant alone and was characterized by neutrophil, mast cell, and eosinophil infiltration. TFA-specific IgG1 antibodies directly correlated with hepatitis, whereas S100 autoantibodies did not. TNF-α, IL-1β, and IL-6 released from splenocytes collected 2 weeks after TFA-S100 inoculation were increased resembling the elevated serum cytokines reported in patients with autoimmune hepatitis (AIH). Three weeks after inoculation, the peak of hepatitis, we noted decreased numbers of Kupffer cells and lower levels of IL-6 and IL-10 in the liver, cytokines produced by Kupffer cells. This is the first report, to our knowledge, of a hapten-induced model of hepatitis with immune and autoimmune features.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental and Molecular Pathology - Volume 78, Issue 2, April 2005, Pages 87-100
Journal: Experimental and Molecular Pathology - Volume 78, Issue 2, April 2005, Pages 87-100
نویسندگان
Dolores B. Njoku, Monica V. Talor, DeLisa Fairweather, Sylvia Frisancho-Kiss, Oludare A. Odumade, Noel R. Rose,