کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9111064 | 1155388 | 2005 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Interferon-γ associated cytokines and chemokines produced by spleen cells from Brucella-immune mice
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موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
علوم غدد
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![عکس صفحه اول مقاله: Interferon-γ associated cytokines and chemokines produced by spleen cells from Brucella-immune mice Interferon-γ associated cytokines and chemokines produced by spleen cells from Brucella-immune mice](/preview/png/9111064.png)
چکیده انگلیسی
It is known that interferon (IFN)-γ plays a critical role in protection against brucellosis. In this study we have investigated several cytokines and chemokines that are associated with IFN-γ for potential in vitro correlates of protection. We cultured spleen cells in vitro from mice immunized orally with a live, attenuated Brucella melitensis vaccine candidate (WR201) and stimulated these cells with a lysate of B. melitensis. Differential gene expression of several cytokines and chemokines in stimulated spleen cells was analysed by real-time PCR, and secreted proteins were determined by ELISA. Immunized mice produced higher levels of both protein and gene transcripts for IFN-γ, interleukin (IL)-2, IL-18 and MIP1-α. Immunized mice also had elevated gene expression levels for IL12-p40, IL23-p19, IP-10, MIG and MCP-1 when compared to normal mice. In this study we have identified new cytokines and chemokines as potential immune correlates in responses to protection in Brucella-vaccinated mice.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cytokine - Volume 30, Issue 2, 21 April 2005, Pages 86-92
Journal: Cytokine - Volume 30, Issue 2, 21 April 2005, Pages 86-92
نویسندگان
Chrysanthi Paranavitana, Elzbieta Zelazowska, Mina Izadjoo, David Hoover,