کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9113156 | 1156213 | 2005 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Dimerizations of the wallaby gonadotropin-releasing hormone receptor and its splice variants
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
علوم غدد
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چکیده انگلیسی
Dimerization between wallaby GnRH-R and its splice variants was examined. A baculovirus-based fluorescence resonance energy transfer (Bv-FRET) assay was used to assess protein-protein interaction between wild type wallaby GnRH-R and splice variants (GnRH-RÎ1 and GnRH-RÎ2). FRET analysis demonstrated that GnRH-R, GnRH-RÎ1 or GnRH-RÎ2 are capable of assembling as homodimers. When GnRH-R is co-expressed with GnRH-RÎ1 or GnRH-RÎ2 splice variants, GnRH-R can form heterodimers with GnRH-RÎ1 and GnRH-RÎ2. GnRH agonist is not required for the initiation of dimerization. However, the addition of a GnRH agonist enhances the FRET signal in the GnRH-R homodimers, indicating that the GnRH agonist may be involved in modulating the extent of dimerization. In addition, this study reveals that dimerization of GnRH-R may be mediated by two or more protein interaction domains. One of them is probably located between amino acid residues 74 and 174, and the other one between residues 175 and 328. This is the first study to show dimerization between a wild type mammalian GnRH-R and its splice variants. It provides additional support for the potential involvement of splice variants in GnRH-R signaling.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: General and Comparative Endocrinology - Volume 144, Issue 3, December 2005, Pages 280-288
Journal: General and Comparative Endocrinology - Volume 144, Issue 3, December 2005, Pages 280-288
نویسندگان
Timothy C. Cheung, John P. Hearn,