کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9121291 | 1159183 | 2005 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Construction of the first shuttle vectors for gene cloning and homologous recombination in Mycoplasma agalactiae
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Construction of the first shuttle vectors for gene cloning and homologous recombination in Mycoplasma agalactiae Construction of the first shuttle vectors for gene cloning and homologous recombination in Mycoplasma agalactiae](/preview/png/9121291.png)
چکیده انگلیسی
Mycoplasma agalactiae is a worldwide ruminant pathogen that causes significant economic losses by inflicting contagious agalactia in sheep and goats. The development of efficient control strategies requires a better understanding of the mycoplasma factors that promote successful infection. However, lack of genetic tools has been a major impediment in studying the pathogenic mechanisms of M. agalactiae. This study describes the identification and cloning of the M. agalactiae origin of replication (oriC) in order to construct the first shuttle vectors for targeted gene disruption, gene complementation and expression studies. Additionally, this report provides the first evidence of the occurrence of homologous recombination and the functionality of heterologous tetM determinant in this pathogen.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEMS Microbiology Letters - Volume 253, Issue 1, 1 December 2005, Pages 89-94
Journal: FEMS Microbiology Letters - Volume 253, Issue 1, 1 December 2005, Pages 89-94
نویسندگان
Rohini Chopra-Dewasthaly, Marc Marenda, Renate Rosengarten, Wolfgang Jechlinger, Christine Citti,