کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
9121667 1159194 2005 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Genotyping of Chromobacterium violaceum isolates by recA PCR-RFLP analysis
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی ژنتیک
پیش نمایش صفحه اول مقاله
Genotyping of Chromobacterium violaceum isolates by recA PCR-RFLP analysis
چکیده انگلیسی
Intraspecies variation of Chromobacterium violaceum was examined by comparative sequence - and by restriction fragment length polymorphism analysis of the recombinase A gene (recA-PCR-RFLP). Primers deduced from the known recA gene sequence of the type strain C. violaceum ATCC 12472T allowed the specific amplification of a 1040 bp recA fragment from each of the 13 C. violaceum strains investigated, whereas other closely related organisms tested negative. HindII-PstI-recA RFLP analysis generated from 13 representative C. violaceum strains enabled us to identify at least three different genospecies. In conclusion, analysis of the recA gene provides a rapid and robust nucleotide sequence-based approach to specifically identify and classify C. violaceum on genospecies level.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEMS Microbiology Letters - Volume 244, Issue 2, 15 March 2005, Pages 347-352
نویسندگان
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