کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
9121679 1159194 2005 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Observation of bias associated with re-amplification of DNA isolated from denaturing gradient gels
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی ژنتیک
پیش نمایش صفحه اول مقاله
Observation of bias associated with re-amplification of DNA isolated from denaturing gradient gels
چکیده انگلیسی
DNA from environmental PCR products separated by denaturing gradient gel electrophoresis (DGGE) was isolated from the background smear rather than from discrete bands of the DGGE gel. The “interband” region was considered as a potential source of less dominant members of natural microbial communities. Surprisingly, instead of detecting new bands from the re-amplified PCR products, patterns very similar to the original ones were obtained regardless of the position of the “interband” region. The results suggest that the separation of amplicons by DGGE may not be perfect and band re-amplification based sequence analyses need careful interpretation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEMS Microbiology Letters - Volume 244, Issue 2, 15 March 2005, Pages 385-390
نویسندگان
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