کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9121995 | 1159202 | 2005 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A fungus-type β-galactofuranan in the cultivated Trebouxia photobiont of the lichen Ramalina gracilis
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موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
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چکیده انگلیسی
A structural characterization of polysaccharides extracted from the aposymbiotically cultured photobiont of the lichen Ramalina gracilis was carried out in order to compare them with those previously found in the symbiotic thallus. The photobiont was isolated from thallus fragments, following the method of Yamamoto, and cultivated in a liquid nutrient medium. Freeze-dried cells were defatted, and the polysaccharides extracted successively with water and aq. 10% KOH, each at 100 °C. After purification, the soluble fractions provided a polysaccharide containing a (1 â 5)-linked β-galactofuranosyl backbone, substituted in a small proportion at O-6 by β-Galf units. Amylose was also found, as insoluble material obtained on freeze-thawing of the alkaline extract. These polysaccharides have not been found in the symbiotic thallus of Ramalina gracilis, which contained only water-soluble (isolichenan) and insoluble glucans (nigeran and laminaran), and galactomannan. Surprisingly, the galactofuranan has similarities with those found in some fungal cell walls.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEMS Microbiology Letters - Volume 244, Issue 1, 1 March 2005, Pages 193-198
Journal: FEMS Microbiology Letters - Volume 244, Issue 1, 1 March 2005, Pages 193-198
نویسندگان
Lucimara M.C. Cordeiro, Elaine R. Carbonero, Guilherme L. Sassaki, Rodrigo A. Reis, Elfriede Stocker-Wörgötter, Philip A.J. Gorin, Marcello Iacomini,