کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9126878 | 1569961 | 2005 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Candidate Sertoli cell specific promoter element for a TGFβ family member (Amh) and a 3ⲠUTR enhancer/repressor for the same gene
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Candidate Sertoli cell specific promoter element for a TGFβ family member (Amh) and a 3â² UTR enhancer/repressor for the same gene Candidate Sertoli cell specific promoter element for a TGFβ family member (Amh) and a 3â² UTR enhancer/repressor for the same gene](/preview/png/9126878.png)
چکیده انگلیسی
Comparison between 400 nt of mouse DNA sequence immediately upstream (5â²) of the coding sequence of the Sertoli cell expressed genes, Amh and Tsx, identified a 33 nt sequence with a significant identity: this was considered to be a candidate Sertoli specific regulatory element (SSRE). Another highly conserved sequence has been identified immediately downstream (3â²) of the Amh polyadenylation signal (DSRE). The action and specificity of these two putative regulatory elements, inserted into a reporter vector (pd2EGFP), has been investigated by transient transfection of mouse fibroblast and Sertoli cells. When combined together or alone, with a minimal thymidine kinase (Tk) promoter, SSRE showed a weak incremental action on the expression of the reporter gene, regardless of the cell type: in contrast there was a 2- to 3-fold decrease when DSRE was present. However, in the absence of Tk there was evidence for a strong synergy between SSRE and DSRE, which was significantly greater in the Sertoli as compared with the fibroblast cells. These results support the view that SSRE exhibits a degree of Sertoli specificity and acts synergistically with DSRE in controlling the expression of Amh.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 363, 19 December 2005, Pages 159-165
Journal: Gene - Volume 363, 19 December 2005, Pages 159-165
نویسندگان
David W. Dresser, Daniel Guerrier,