کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9126936 | 1569965 | 2005 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Identification of cold acclimated genes in leaves of Citrus unshiu by mRNA differential display
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کلمات کلیدی
oligodeoxyribonucleotide thymidineamino acid permeaseAFLPdeoxyribonucleaseCBFdNTPM-MuLVDDRT-PCROligo(dT)RNasecDNA - cDNADNA complementary to RNA - DNA مکمل RNADNAse - DNAaseCold acclimation - انعطاف پذیری سردGene expression - بیان ژنSerial analysis of gene expression - تجزیه و تحلیل سریال بیان ژنReverse transcriptase - ترانس کریپتاز معکوس یا وارونویسSAGE - حکیمdeoxyribonucleoside triphosphate - دز اکسید ریبونوکلئوزید تری فسفاتNucleoside diphosphate kinase - دی اکسید کیناز دی اکسید کربنribonuclease - ریبونوکلئازMoloney murine leukemia virus - ویروس لوئی موش Moloney14-3-3 protein - پروتئین 14-3-3Amplified fragment length polymorphism - پلی مورفیسم طول قطعه تقویت شده
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
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چکیده انگلیسی
Citrus unshiu is freeze tolerant to â 10 °C when fully acclimated after exposure to cold, nonfreezing temperatures. To gain an understanding of its cold tolerance mechanism, mRNA differential display reverse transcriptase polymerase chain reaction (DDRT-PCR) and quantitative relative RT-PCR were used to study gene expression under a gradual cold-acclimation temperature regime. Six up-regulated and two down regulated genes were identified based on their amino acid sequences. The identified proteins encoded by the up-regulated genes were: 14-3-3 protein, 40S ribosomal protein S23, putative 60S ribosomal protein L15, nucleoside diphosphate kinase III protein, regulator of chromosome condensation-like protein, and amino acid permease 6. The proteins encoded by the two down-regulated genes were: miraculin-like protein and beta-galactosidase. Their individual function has been briefly reviewed based on published information. In addition to the findings in this study, we compared the function of cold responsive genes of Poncirus trifoliata, a very cold hardy relative of Citrus species that is freeze tolerant to â 30 °C when fully acclimated, to the function of genes in the current study.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 359, 10 October 2005, Pages 111-118
Journal: Gene - Volume 359, 10 October 2005, Pages 111-118
نویسندگان
Ping Lang, Can-kui Zhang, Robert C. Ebel, Fenny Dane, William A. Dozier,