کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
9127088 1569970 2005 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
PU.1-mediated transcriptional regulation of prophenin-2 in primary bone marrow cells
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی ژنتیک
پیش نمایش صفحه اول مقاله
PU.1-mediated transcriptional regulation of prophenin-2 in primary bone marrow cells
چکیده انگلیسی
Prophenin-2 (PF-2) is a cathelicidin, 97-amino-acid antimicrobial protein stored in neutrophil secondary granules. PF-2 is expressed specifically in porcine immature myeloid cells; however, little is known about its regulation. In this study, we characterized the 5′ regulatory regions of the PF-2 gene to understand the molecular mechanisms regulating its expression. Using bioinformatic approaches, site-directed mutagenesis, and transactivation experiments, we found that the PF-2 gene was regulated by transcription factor PU.1. In addition, PF-2 expression also is regulated by the cytokines GM-CSF and IL-3. Taken together, these results identify cis- and trans-acting factors involved in the regulation of PF-2 and clarify mechanisms of cathelidicin gene regulation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 352, 6 June 2005, Pages 1-9
نویسندگان
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