کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9127110 | 1569970 | 2005 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The silk moth Bombyx mori U1 and U2 snRNA variants are differentially expressed
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کلمات کلیدی
snRNAsnRNAssnRNPRT-PCRWGSsmall nuclear RNA - RNA هسته ای کوچکBasic Local Alignment Search Tool - ابزار جستجوی محلی سازگاری محلیSpliceosome - اسپلیسئوزومBlast - انفجارdifferential expression - بیان دیفرانسیلwhole-genome shotgun - تفنگ کامل ژنومInsect - حشرهsmall nuclear ribonucleoprotein - ریبونولوپروتئین کوچک هسته ایSilk gland - غده طلاییreverse transcriptase polymerase chain reaction - واکنش زنجیره ای پلی مراز ترانس کریتاز معکوسpolymerase chain reaction - واکنش زنجیره ای پلیمرازPCR - واکنش زنجیرهٔ پلیمرازBombyx mori - کرم ابریشم
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Five U1 and eight U2 isoforms of the silk moth Bombyx mori exhibiting internal nucleotide differences have been previously identified and characterized in various tissues and developmental stages. In this investigation, it is demonstrated that the levels of some snRNA variants differ in egg and silk gland tissue and change during development. Qualitative and quantitative differences in the U1 and U2 variant populations were observed at three developmental points (early, middle and late) of the silk gland (SG) during the fifth instar larval stage of the silk moth. Statistical analyses of the various isoform populations across the fifth instar larval and egg stages show significant differences for some of the U1 and U2 variants. The representation of variant sequences in expressed U1 and U2 sequences (RT-PCR libraries) and in a whole-genome shotgun (WGS) assembly database was confirmed. In addition, conserved elements in the promoter 5â²-flanking region of the U1 and U2 variants were identified in the WGS.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 352, 6 June 2005, Pages 127-136
Journal: Gene - Volume 352, 6 June 2005, Pages 127-136
نویسندگان
Julie M. Sierra-Montes, Simone Pereira-Simon, Shamayra S. Smail, Rene J. Herrera,