کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
9127121 1569972 2005 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Development of a spot reliability evaluation score for DNA microarrays
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی ژنتیک
پیش نمایش صفحه اول مقاله
Development of a spot reliability evaluation score for DNA microarrays
چکیده انگلیسی
We developed a reliability index named SRED (Spot Reliability Evaluation Score for DNA microarrays) that represents the probability that the calibrated gene expression level from a DNA microarray would be less than a factor of 2 different from that of quantitative real-time polymerase chain reaction assays whose dynamic quantification range is treated statistically to be similar to that of the DNA microarray. To define the SRED score, two parameters, the reproducibility of measurement value and the relative expression value were selected from nine candidate parameters. The SRED score supplies the probability that the expression level in each spot of a microarray is less than a certain-fold different compared to other expression profiling data, such as QRT-PCR. This score was applied to ∼1,500,000 points of the expression profile in the RIKEN Expression Array Database.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 350, Issue 2, 9 May 2005, Pages 149-160
نویسندگان
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