کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9127140 | 1160201 | 2005 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Virus-based reporter systems for monitoring transcriptional activity of hypoxia-inducible factor 1
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کلمات کلیدی
LTRENO1Enolase 1von Hippel Lindau proteinlacZLDHAONPGpVHL5-bromo-4-chloro-3-indolyl β-d-galactopyranosideX-GalMCMVHREDesferrioxamine mesylatedsDNADFOHIF-1o-nitrophenyl β-D-galactopyranosideβgalPGK1double stranded DNA - DNA رشته ایSmall interfering RNA - RNA تداخل کوچکsiRNA - siRNAβ-galactosidase - بتا گالاکتوزیدازsin - بدونLong terminal repeat - تکرار طولانی مدتBase pair(s) - جفت پایه (ها)hour(s) - ساعت ها)hypoxia-inducible factor 1 - عامل القایی هیپوکسی 1hypoxia response element - عنصر پاسخ هیپوکسیVascular endothelial growth factor - فاکتور رشد اندوتلیال عروقیVascular Endothelial Growth Factor (VEGF) - فاکتور رشد اندوتلیال عروقی (VEGF)Phosphoglycerate kinase 1 - فسفوگلیسرات کیناز 1Lactate dehydrogenase A - لاکتات دهیدروژناز Anucleotide(s) - نوکلئوتید (ها)unit(s) - واحد (ها)HIV-1 - ویروس اچ آی وی نوع یکHuman immunodeficiency virus type 1 - ویروس نقص ایمنی بدن نوع 1β-galactosidase gene - ژن β-گالاکتوزیداز
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Being key regulator of oxygen homeostasis hypoxia-inducible factor 1 (HIF-1) plays significant roles in cancer progression as well as in cardiovascular diseases. The modulation of HIF-1α activity in vivo may represent a valuable therapeutic approach to these disorders [Hofer, T., Desbaillets, I., Hopfl, G., Wenger, R.H., Gassmann, M., 2002. Characterization of HIF-1 alpha overexpressing HeLa cells and implications for gene therapy. Comp. Biochem. Physiol., Toxicol. Pharmacol. 133, 475-481]. In order to monitor HIF-1 transcriptional activity, we have developed HIF-1α-responsive reporter constructs, in which lacZ gene expression is driven by minimal Hsp70 gene promoter or minimal immediate early promoter of cytomegalovirus (CMV) and a combination of hypoxia response elements from regulatory regions of PGK1, ENO1 and LDHA genes. For the efficient delivery to a wide variety of cell types we chose retroviral and lentiviral vectors as carriers of the reporter cassette. We demonstrate that the obtained reporter system i) has a high inducibility in response to treatments leading to HIF-1α activation, ii) shows upregulation in response to HIF-1 activation and downregulation following inhibition of HIF-1α expression by small interfering RNA, iii) follows the dynamics of endogenous HIF-1 target gene expression. The retrovirus- and lentivirus-based reporters can be used for high-throughput screening of HIF-1α modulators and for the study of crosstalk between HIF-1 and different related signal transduction pathways. Potential applications for the reporters are discussed.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 350, Issue 1, 25 April 2005, Pages 89-98
Journal: Gene - Volume 350, Issue 1, 25 April 2005, Pages 89-98
نویسندگان
O.V. Razorenova, A.V. Ivanov, A.V. Budanov, P.M. Chumakov,