کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9127358 | 1569976 | 2005 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Expression of the mouse WNK1 gene in correlation with ganglioside GD3 and functional analysis of the mouse WNK1 promoter
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کلمات کلیدی
GangliosideAMVcDNA - cDNADNA complementary to mRNA - DNA مکمل به mRNAEST - استGene expression - بیان ژنReverse transcriptase - ترانس کریپتاز معکوس یا وارونویسTranscriptional regulation - تنظیم ترانزیتیExpressed Sequence Tag - تکرار اظهار نظرbase pairs - جفت پایهpolymerase chain reaction - واکنش زنجیره ای پلیمرازPCR - واکنش زنجیرهٔ پلیمرازProtein kinase - پروتئین کینازPromoter - پروموترkilobases - کیلوبا ها
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Expression of the mouse WNK1 gene in correlation with ganglioside GD3 and functional analysis of the mouse WNK1 promoter Expression of the mouse WNK1 gene in correlation with ganglioside GD3 and functional analysis of the mouse WNK1 promoter](/preview/png/9127358.png)
چکیده انگلیسی
WNK1 is one of WNK (With No K=Lysine) protein kinases which comprise a newly described subfamily. Our studies showed that expression of the mouse WNK1 gene was dramatically suppressed in a tumor cell line when its phenotype was altered by suppression of the GD3-synthase gene expression. The mouse WNK1 gene was expressed at a high level at early stage of embryonic brain and its expression decreased as brain developed, similar to the expression pattern of the GD3-synthase gene. To study transcriptional regulation, we cloned a 5â²-flanking 1239-bp fragment of the mouse WNK1 gene. This fragment contains a number of potential consensus binding sites for transcription factors, including Sp1, AP2, CCAAT, Est-1, Oct-1, CNBP, and NFkB, but lacks a TATA box. Primer extension identified multiple putative transcriptional initiation sites, including several sites downstream of the ATG codon. Activities of the promoter fragments were assessed in mouse breast Sa/R-MT cells by transient transfection and the results showed that the promoter elements between â700 and â977 is required for maintaining a high level of promoter activity of the TATA-less mouse WNK1 gene.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 344, 3 January 2005, Pages 233-239
Journal: Gene - Volume 344, 3 January 2005, Pages 233-239
نویسندگان
Guichao Zeng, Luoyi Gao, Tian Xia, Yihua Gu, Robert K. Yu,