کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9139959 | 1163387 | 2005 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The two cytoplasmic dynein-2 isoforms in Leishmania mexicana perform separate functions
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کلمات کلیدی
PFRAxonemeLMXIFTDynein heavy chainKinetoplastid - kinetoplastidintraflagellar transport - حمل داخل قطارdynein - دینینLeishmania - لیشمانیا Leishmania mexicana - لیشمانیا مکزیکیParaflagellar rod - میله پارافلاژلارpolymerase chain reaction - واکنش زنجیره ای پلیمرازPCR - واکنش زنجیرهٔ پلیمرازFlagellum - پرچم
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شناسی مولکولی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: The two cytoplasmic dynein-2 isoforms in Leishmania mexicana perform separate functions The two cytoplasmic dynein-2 isoforms in Leishmania mexicana perform separate functions](/preview/png/9139959.png)
چکیده انگلیسی
Eukaryotic organisms with cilia or flagella typically express two non-axonemal or “cytoplasmic” dyneins, dynein-1 and dynein-2. Interestingly, we find that Leishmania mexicana is unusual and contains two distinct cytoplasmic dynein-2 heavy chain genes (designated LmxDHC2.1 and LmxDHC2.2) along with a single dynein-1 heavy chain (LmxDHC1). Disruption of LmxDHC2.2 resulted in immotile parasites that had a rounded cell body. Although they assume amastigote morphology, immunoblot analysis of these cells demonstrates protein expression consistent with the promastigote stage. Ultrastructural analysis revealed non-emergent flagella that lacked the paraflagellar rod and an axoneme with deficiencies in several components. We confirmed the absence of paraflagellar rod proteins PFR1 and PFR2. These results show that LmxDHC2.2 is required for flagellar assembly and also participates in the maintenance of promastigote cell shape. In contrast to the results with LmxDHC2.2, we were unable to generate homologous disruptions of LmxDHC2.1. This result suggests that, unlike LmxDHC2.2, LmxDHC2.1 is an essential gene in Leishmania. Together, these findings demonstrate that the two dynein-2 heavy chain isoforms in Leishmania perform distinct functions. The observation that the genomes of Leishmania major, Leishmania infantum and Trypanosoma brucei also contain two dynein-2 isoforms suggests that this unusual aspect of cytoplasmic dynein is a conserved feature of the kinetoplastids.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular and Biochemical Parasitology - Volume 143, Issue 2, October 2005, Pages 216-225
Journal: Molecular and Biochemical Parasitology - Volume 143, Issue 2, October 2005, Pages 216-225
نویسندگان
Christine Adhiambo, James D. Forney, David J. Asai, Jonathan H. LeBowitz,