کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9139973 | 1163389 | 2005 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Toxoplasma gondii is capable of exogenous folate transport
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کلمات کلیدی
HFFBT1dihydrofolate reductase-thymidylate synthaseDHFR-TSMTXMWCOORFPBSFolic acid - اسید فولیکMembrane transporter - حمل کننده غشاییhuman foreskin fibroblast - فیبروبلاست خارجی پوست انسانopen reading frame - قاب خواندن بازFolate metabolism - متابولیسم فولاتMethotrexate - متوتروکساتPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریApicomplexa - هاگ داران، اپیکامپلکساMolecular weight - وزن مولکولیMolecular Weight Cut Off - وزن مولکولی کاهش یافته استintracellular parasitism - پارازیتیسم داخل سلولی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شناسی مولکولی
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چکیده انگلیسی
Folates are key elements in eukaryotic biosynthetic processes. The protozoan parasite Toxoplasma gondii possesses the enzymes necessary for de novo folate synthesis and has been suggested to lack alternative mechanisms for folate acquisition. In this paper, we present a different view by providing evidence that Toxoplasma is capable of salvaging exogenous folates. By monitoring uptake of radiolabeled folates by parasites in axenic conditions, our studies revealed a common folate transporter that has a high affinity for folic acid. Transport of this compound across the parasite plasma membrane is rapid, biphasic, temperature dependent, bi-directional, concentration dependent and specific. In addition, morphological evidence demonstrates that fluorescent methotrexate, a folate analog, is internalized by Toxoplasma and shows localization reminiscent to the mitochondrion. The presence of putative folate transporter genes in the Toxoplasma genome, which are homologous to the BT1 family of proteins, suggests that Toxoplasma may encode proteins involved in folate transport. Interestingly, genome analysis suggests that the BT1 family of proteins exists not only in Toxoplasma, but in other Apicomplexan parasites as well. Altogether, our results not only have implications for current therapeutic regimens against T. gondii, but they also allude that the folate transport mechanism may represent a novel Apicomplexan target for the development of new drugs.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular and Biochemical Parasitology - Volume 144, Issue 1, November 2005, Pages 44-54
Journal: Molecular and Biochemical Parasitology - Volume 144, Issue 1, November 2005, Pages 44-54
نویسندگان
Kristen M. Massimine, Lanxuan T. Doan, Chloé A. Atreya, Timothy T. Stedman, Karen S. Anderson, Keith A. Joiner, Isabelle Coppens,