کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9140034 | 1163393 | 2005 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Organelle-specific cochaperonins in apicomplexan parasites
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کلمات کلیدی
MTGGFPACPRBCChaperoneDsRedMitoTracker GreenTransfection - ترانسفکشنMitochondrion - میتوکندریونApicomplexa - هاگ داران، اپیکامپلکساpolymerase chain reaction - واکنش زنجیره ای پلیمرازPCR - واکنش زنجیرهٔ پلیمرازacyl carrier protein - پروتئین حامل آکیلgreen fluorescent protein - پروتئین فلورسنت سبزPlastid - پلاستیدhuman red blood cell - گلبولهای قرمز خون انسان
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شناسی مولکولی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Organelle-specific cochaperonins in apicomplexan parasites Organelle-specific cochaperonins in apicomplexan parasites](/preview/png/9140034.png)
چکیده انگلیسی
Protein maturation in eukaryotic organelles requires the type I chaperonin system; this comprises chaperonin 60 (Cpn60) and its cochaperonin. We have re-examined and revised the sequence of the nuclear genes specifying organellar cochaperonins in Plasmodium falciparum (Pf). One gene encodes a typical cochaperonin (PfCpn10) whereas the other (encoding PfCpn20) specifies two Cpn10 domains arranged in tandem as in plant chloroplasts. Transfection experiments using fluorescent reporters showed specific localization of PfCpn10 to the mitochondrion and PfCpn20 to the plastid. As P. falciparum also has two Cpn60s, one of which is targeted specifically to the mitochondrion and the other exclusively to the plastid, each organelle has a distinct type I chaperonin system. Comparative sequence analysis extended these findings to several other apicomplexan parasites that have both a mitochondrion and a plastid. Phylogenetic analysis suggests the Cpn10s and Cpn20s of apicomplexans are independently monophyletic. The apicomplexan Cpn10 is phylogenetically related to other mitochondrial versions but a significant relationship between apicomplexan Cpn20s and other cochaperonins was not established.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular and Biochemical Parasitology - Volume 141, Issue 2, June 2005, Pages 133-143
Journal: Molecular and Biochemical Parasitology - Volume 141, Issue 2, June 2005, Pages 133-143
نویسندگان
Shigeharu Sato, R.J.M. (Iain) Wilson,