کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9140049 | 1570661 | 2005 | 14 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Recombinant and native Plasmodium falciparum TATA-binding-protein binds to a specific TATA box element in promoter regions
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کلمات کلیدی
TBPPICKAHRPEMSAElectrophoretic mobility shift assay - آزمون تحرک تحرک الکتروفورزchromatin immunoprecipitation - ایمن سازی کروماتینTATA box - جعبه TATAknob-associated histidine-rich protein - پروتئین غنی از هیستیدین همراه با دستگیرهPlasmodium falciparum - پلاسمودیوم فالسیپارومpreinitiation complex - پیچیدگی preinitiationCHiP - چیپ
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شناسی مولکولی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Recombinant and native Plasmodium falciparum TATA-binding-protein binds to a specific TATA box element in promoter regions Recombinant and native Plasmodium falciparum TATA-binding-protein binds to a specific TATA box element in promoter regions](/preview/png/9140049.png)
چکیده انگلیسی
RNA polymerase II promoters in Plasmodium spp., like in most eukaryotes, have a bipartite structure. However, the identification of a functional TATA box located within the Plasmodium spp. core promoters has been difficult, mainly because of its high AÂ +Â T content. Only few putative trans-acting elements have been identified in the malaria parasite genome such as a gene orthologous to the TATA box binding protein (PfTBP). In this study, we demonstrate that PfTBP is part of the DNA-protein complexes formed in the kahrp and gbp-130 gene promoter regions. Supershift and footprinting assays performed with a GST-PfTBP fusion protein showed that PfTBP associates with a consensus TATA box sequence located 81 base pairs upstream of the transcription start site in the kahrp promoter region and with a TATA box-like (TGTAA) sequence at position â186 of the gbp-130 gene promoter region. Chromatin immunoprecipitation assays confirmed that native PfTBP is able to associate in vivo with both TATA box elements. This is the first study that reports the identification of cis-acting sequences (TATAA and TGTAA) and their corresponding trans-acting (PfTBP) factor in P. falciparum.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular and Biochemical Parasitology - Volume 140, Issue 2, April 2005, Pages 183-196
Journal: Molecular and Biochemical Parasitology - Volume 140, Issue 2, April 2005, Pages 183-196
نویسندگان
Omar K. Ruvalcaba-Salazar, Ma. del Carmen RamÃrez-Estudillo, Dvorak Montiel-Condado, Félix Recillas-Targa, Miguel Vargas, Rosaura Hernández-Rivas,