کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9142042 | 1163894 | 2005 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A type II phosphatidylinositol 4-kinase associates with T cell receptor ζ chain in Con A stimulated splenic lymphocytes through tyrosyl phosphorylation-dependent mechanisms
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شناسی مولکولی
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چکیده انگلیسی
T cells show rapid reorganization of cytoskeleton in response to antigenic stimulation. The molecular mechanisms by which TCR-CD3 regulates actin cytoskeleton are not well defined. Here we show that a type II PtdIns 4-kinase associates with cytoskeletal fraction in splenic lymphocytes in response to Con A. Protein tyrosyl phosphorylation of type II PtdIns 4-kinase appears to be the mechanism for its association with cytoskeleton. Over-lay blots suggest that the enzyme binds to TCR-CD3 ζ chain in the cytoskeletal fraction. Anti-TCR-CD3 ζ antibodies competitively inhibit PtdIns 4-kinase association with TCR-CD3 ζ chain. Immunodepletion of TCR-CD3 ζ decreases PtdIns 4-kinase activity in the cytoskeletal fraction with a concomitant increase in PtdIns 4-kinase activity in anti-TCR-CD3 ζ immunoprecipitates. We propose that the association of type II PtdIns 4-kinase with TCR-CD3 ζ chain may bring the enzyme into close proximity of actin and a possible regulation of actin polymerization through localized production of PtdIns4P and PtdIns(4,5)P2.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular Immunology - Volume 42, Issue 5, March 2005, Pages 561-568
Journal: Molecular Immunology - Volume 42, Issue 5, March 2005, Pages 561-568
نویسندگان
Aaron Z. Fernandis, Rupa Srivastava, Ranjeet K. Sinha, Gosukonda Subrahmanyam,