Temporal changes in cellular energy following burn injury

Availability of ADP is a predominant influence on respiratory control. Associated with severe burn injury is an increase in energy expenditure. The purpose of this study was to determine the temporal changes in ATP, ADP, NAD, and NADH following severe burn and thereby assess any related alterations in respiratory control and energy deficit. During isoflurane anesthesia and following intraperitoneal injection of saline, 32 mice were flame burned at 40% body surface area. Twelve mice served as controls. At 12, 24, 72, and 168 h post-burn, groups of mice underwent celiotomy with determination of hepatic surface blood flow using laser Doppler and oxygen saturation using pulse oximetry. Biopsies of liver were then frozen in liquid nitrogen for subsequent quantification of ATP, ADP, AMP, NAD, and NADH by HPLC. Mortality was 12.5% at 72 h post-burn and 25% at 1 week. Oxygen saturation and hepatic surface blood flow remained similar to control values throughout the week after burn. ATP, ADP, and energy charge decreased progressively following burn reaching a significant decrease from unburned controls at 72 h. Availability of NADH remained statistically similar to unburned controls throughout the week after burn. These results demonstrate that despite maintenance of baseline oxygen delivery, there was a nadir in ATP and ADP availability and energy charge in the liver at 72 h after burn. This finding supports the concept of a limitation in phosphorylation after injury. Availability of NADH remained at or above pre-burn concentrations suggesting that the rate of fuel oxidation was not a limiting factor for ongoing oxidative phosphorylation for energy.