کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9244222 | 1209904 | 2005 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Connective Tissue Growth Factor (CCN2) in Rat Pancreatic Stellate Cell Function: Integrin α5β1 as a Novel CCN2 Receptor
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کلمات کلیدی
HSCTGF-βCCN2HSPGPDGFPSCHRPFBSDMEMα-SMABSA - BSADulbecco’s modified Eagle medium - Modified Eagle اصلاح شده Dulbeccobovine serum albumin - آلبومین سرم گاوα–smooth muscle actin - اکتین عضله آلفا صافTransforming growth factor β - تبدیل فاکتور رشد βfetal bovine serum - سرم جنین گاوHepatic stellate cell - سلول ستاره ای کبدیpancreatic stellate cell - سلول ستون لوزالمعدهConnective tissue growth factor - فاکتور رشد بافت همبندplatelet-derived growth factor - فاکتور رشد حاصل از پلاکتFibronectin - فیبرونکتینHeparan sulfate proteoglycan - هپارین سولفات پروتئگلیکانHorseradish peroxidase - پراکسیداز هوررادیش
موضوعات مرتبط
علوم پزشکی و سلامت
پزشکی و دندانپزشکی
بیماریهای گوارشی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Connective Tissue Growth Factor (CCN2) in Rat Pancreatic Stellate Cell Function: Integrin α5β1 as a Novel CCN2 Receptor Connective Tissue Growth Factor (CCN2) in Rat Pancreatic Stellate Cell Function: Integrin α5β1 as a Novel CCN2 Receptor](/preview/png/9244222.png)
چکیده انگلیسی
Background & Aims: Pancreatic stellate cells (PSCs) are proposed to play a key role in the development of pancreatic fibrosis. The aim of this study was to evaluate the production by rat activated PSCs of the fibrogenic protein, connective tissue growth factor (CCN2), and to determine the effects of CCN2 on PSC function. Methods: CCN2 production was evaluated by immunoprecipitation and promoter activity assays. Expression of integrin α5β1 was examined by immunoprecipitation and Western blot. Binding between CCN2 and integrin α5β1 was determined in cell-free systems. CCN2 was assessed for its stimulation of PSC adhesion, migration, proliferation, DNA synthesis, and collagen I synthesis. Results: CCN2 was produced by activated PSCs, and its levels were enhanced by transforming growth factor β1 treatment. CCN2 promoter activity was stimulated by transforming growth factor β1, platelet-derived growth factor, alcohol, or acetaldehyde. CCN2 stimulated integrin α5β1-dependent adhesion, migration, and collagen I synthesis in PSCs. Integrin α5β1 production by PSCs was verified by immunoprecipitation, while direct binding between integrin α5β1 and CCN2 was confirmed in cell-free binding assays. Cell surface heparan sulfate proteoglycans functioned as a partner of integrin α5β1 in regulating adhesion of PSCs to CCN2. PSC proliferation and DNA synthesis were enhanced by CCN2. Conclusions: PSCs synthesize CCN2 during activation and after stimulation by profibrogenic molecules. CCN2 regulates PSC function via cell surface integrin α5β1 and heparan sulfate proteoglycan receptors. These data support a role for CCN2 in PSC-mediated fibrogenesis and highlight CCN2 and its receptors as potential novel therapeutic targets.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gastroenterology - Volume 129, Issue 3, September 2005, Pages 1019-1030
Journal: Gastroenterology - Volume 129, Issue 3, September 2005, Pages 1019-1030
نویسندگان
Runping Gao, David R. Brigstock,