کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9244289 | 1209910 | 2005 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Recombinant Probiotics for Treatment and Prevention of Enterotoxigenic Escherichia coli Diarrhea
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کلمات کلیدی
LPSSTXLNTCD50CTXGlcGlcNAcSTECETECenterotoxigenic Escherichia coli - Esterichia coli Enterotoxigenicheat-labile enterotoxin - انتروتوکسین گرم لابلیShiga toxin - سم شیگاcholera toxin - سوزش گوارشیLacto-N-neotetraose - لاکتو N-neotetraoseLuria-Bertani - لواریا بارتانیlipopolysaccharide - لیپوپلی ساکاریدN-acetylglucosamine - نیتستیگلوکوزامینpolymerase chain reaction - واکنش زنجیره ای پلیمرازPCR - واکنش زنجیرهٔ پلیمرازGal - گالGalactose - گالاکتوزمی Glucose - گلوکز
موضوعات مرتبط
علوم پزشکی و سلامت
پزشکی و دندانپزشکی
بیماریهای گوارشی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Background & Aims: We have developed a therapeutic strategy for gastrointestinal infections that is based on molecular mimicry of host receptors for bacterial toxins on the surface of harmless gut bacteria. The aim of this study was to apply this to the development of a recombinant probiotic for treatment and prevention of diarrheal disease caused by enterotoxigenic Escherichia coli strains that produce heat-labile enterotoxin. Methods: This was achieved by expressing glycosyltransferase genes from Neisseria meningitidis or Campylobacter jejuni in a harmless Escherichia coli strain (CWG308), resulting in the production of a chimeric lipopolysaccharide capable of binding heat-labile enterotoxin with high avidity. Results: The strongest heat-labile enterotoxin binding was achieved with a construct (CWG308:pLNT) that expresses a mimic of lacto-N-neotetraose, which neutralized â¥93.8% of the heat-labile enterotoxin activity in culture lysates of diverse enterotoxigenic Escherichia coli strains of both human and porcine origin. When tested with purified heat-labile enterotoxin, it was capable of adsorbing approximately 5% of its own weight of toxin. Weaker toxin neutralization was achieved with a construct that mimicked the ganglioside GM2. Preabsorption with, or coadministration of, CWG308:pLNT also resulted in significant in vivo protection from heat-labile enterotoxin-induced fluid secretion in rabbit ligated ileal loops. Conclusions: Toxin-binding probiotics such as those described here have considerable potential for prophylaxis and treatment of enterotoxigenic Escherichia coli-induced travelers' diarrhea.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gastroenterology - Volume 128, Issue 5, May 2005, Pages 1219-1228
Journal: Gastroenterology - Volume 128, Issue 5, May 2005, Pages 1219-1228
نویسندگان
Adrienne W. Paton, Michael P. Jennings, Renato Morona, Hui Wang, Antonio Focareta, Louise F. Roddam, James C. Paton,