کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
9252 623 2009 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
In vitro behavior of neural stem cells in response to different chemical functional groups
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
In vitro behavior of neural stem cells in response to different chemical functional groups
چکیده انگلیسی

Neural stem cells (NSCs) cultured on glass surfaces modified by different chemical groups, including hydroxyl (–OH), sulfonic (–SO3H), amino (–NH2), carboxyl (–COOH), mercapto (–SH) and methyl (–CH3) groups, are shown here to commit to phonotypes with extreme sensitivity to surface chemical groups. The adhering NSCs at the level of single cells exhibited morphological changes in response to different chemical groups. NSCs on –SO3H surfaces had the largest contact area and the most flattened morphology, while those on –CH3 surfaces exhibited the smallest contact area and the most rounded morphology. After 5 days of culture, the migration of NSCs from their original aggregates onto these test surfaces followed the trend: –NH2>–COOH = –SH≫–SO3H>–CH3>–OH. The expression of specific markers, including nestin, β-Tubulin-III, glial fibrillary acidic protein and O4, were used to examine NSCs lineage specification. The –SO3H surfaces favored NSCs differentiation into oligodendrocytes, while NSCs in contact with –COOH, –NH2, –SH and –CH3 had the ability to differentiate into neurons, astrocytes and oligodendrocytes. Compared to –COOH surfaces, –NH2 seemed to promote neuronal differentiation. These chemically modified surfaces exhibited regulation of NSCs on adhesion, migration and differentiation potential, providing chemical means for the design of biomaterials to direct NSCs lineage specification for neural tissue engineering.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biomaterials - Volume 30, Issue 6, February 2009, Pages 1036–1044
نویسندگان
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