کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
9255 623 2009 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The geometric control of E14 and R1 mouse embryonic stem cell pluripotency by plasma polymer surface chemical gradients
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
The geometric control of E14 and R1 mouse embryonic stem cell pluripotency by plasma polymer surface chemical gradients
چکیده انگلیسی

Plasma polymer surfaces were fabricated such that the cell response to a range of carboxylic acid concentrations on a single sample could be investigated. Surface chemical gradients from hydrophobic plasma polymerised octadiene (OD) to a more hydrophilic plasma polymerised acrylic acid (AA) were formed on glass coverslips. Surface characterisation of the chemical gradients was performed using X-ray photoelectron spectroscopy to determine elemental composition. Following culture of E14 and R1 mouse embryonic stem cells (mES) in differing culture media, cell pluripotency was determined by alkaline phosphatase staining. The results demonstrate that for these cell lines the capacity for self-renewal is maintained if the cells are restricted in their spreading to <120 μm2.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biomaterials - Volume 30, Issue 6, February 2009, Pages 1066–1070
نویسندگان
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