Detection of rubella immunoglobulin M from dried venous blood spots using a commercial enzyme immunoassay

Optical densities were corrected for kit variation as recommended by the manufacturer, but no further adjustment was needed to compare serum and DVB results. Counting equivocal results as positive, the sensitivity of the DVB compared with serum for the categorization of rubella IgM as positive or negative was 96.7% (95% confidence interval [CI], 83.3-100%) and the specificity was 100% (95% CI, 93.7-100%).