کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
9329 626 2008 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Cationic lipid bilayer coated gold nanoparticles-mediated transfection of mammalian cells
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
Cationic lipid bilayer coated gold nanoparticles-mediated transfection of mammalian cells
چکیده انگلیسی

Here, we demonstrated dimethyldioctadecylammonium bromide (DODAB), a cationic lipid, bilayer coated Au nanoparticles (AuNPs) could efficiently deliver two types of plasmid DNA into human embryonic kidney cells (HEK 293) in the presence of serum. The transfection efficiency of AuNPs was about five times higher than that of DODAB. The interaction of AuNPs with DNA was characterized with dye intercalation assay and agarose gel electrophoresis. The morphology of the complex of AuNPs with DNA was observed with scanning electron microscope (SEM). The intracellular trafficking of the complex was monitored with transmission electron microscope (TEM). Based on experimental results, the possible mechanism was proposed and the barriers in the process of transfection were discussed. This work demonstrates a simple way to increase the transfection efficiency of cationic lipid through changing the stability of the complex of cationic lipid with DNA. It may provide some insights into understanding and controlling the interaction of cationic lipid with DNA. It also provides a novel way to construct gold nanoparticles-based gene vectors and some insights into learning the process of nanomaterials-mediated transfection.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biomaterials - Volume 29, Issue 26, September 2008, Pages 3617–3624
نویسندگان
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