Antigenic epitope for sperm-immobilizing antibody detected in infertile women

CD52 is a glycosylphosphatidylinositol (GPI) anchor protein occurring in lymphocytes, the epididymis, seminal plasma and on ejaculated sperm surface. The molecular structure of male reproductive tract CD52 (mrtCD52) is quite different from that of lymphocyte CD52 except for a peptide sequence comprising 12 amino acids. The carbohydrate molecule may thus provide antigenic epitopes to females. The repeated N-acetyllactosamine units of the N-linked carbohydrate present in the mrtCD52 were identified as a pathogenic antigen for infertility using a monoclonal antibody (MAb H6-3C4) generated from an infertile patient's peripheral blood lymphocyte. We suggested also the possible presence of O-linked carbohydrate in human mrtCD52, although it is not known whether this carbohydrate is immunogenic or not. This article gives an overview of the structure and function of mrtCD52 as well as the fertilization-blocking mechanism of the antibody against this molecule.