کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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936962 | 1475210 | 2008 | 9 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Enhancing memory formation by altering protein phosphorylation balance Enhancing memory formation by altering protein phosphorylation balance](/preview/png/936962.png)
In Lymnaea, aerial respiration can be operantly conditioned and depending on the training procedure employed two forms of memory can result: intermediate-term (ITM) and long-term memory (LTM). ITM, which persists for 3 h, is dependent on de novo protein synthesis whilst LTM, which persists for at least 24 h, is dependent on both de novo protein synthesis and altered gene activity. A single 0.5 h training session (i.e. ITM-training) leaves behind a residual molecular memory trace, which a second bout of ITM-training can activate and boost it to a LTM. Here we extend this finding to show that either inhibiting protein phosphatase activity with okadaic acid (1 μM), or increasing protein kinase C (PKC) activity and therefore protein phosphorylation with bryostatin (0.25 ng/mL) treatment prior to ITM-training, results in a LTM. However, following right pedal dorsal 1 (RPeD1) soma ablation neither of these treatments are effective in producing LTM following ITM-training, indicating transcription is a necessity. These findings suggest that the balance between phosphorylation and dephosphorylation in neurons is a key factor for LTM formation.
Journal: Neurobiology of Learning and Memory - Volume 90, Issue 3, October 2008, Pages 544–552