کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
9410778 1613317 2005 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Interleukin-1β induces CREB-binding protein (CBP) mRNA in brain and the sequencing of rat CBP
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب سلولی و مولکولی
پیش نمایش صفحه اول مقاله
Interleukin-1β induces CREB-binding protein (CBP) mRNA in brain and the sequencing of rat CBP
چکیده انگلیسی
Interleukin-1 beta (IL-1) and CREB have many CNS actions including sleep regulation and hippocampal-dependent learning. CREB acts in part via CREB-binding protein (CBP). We thus determined whether IL-1 could induce CBP gene expression. Initially, cultured hippocampal cells were treated with IL-1 and differential display reverse transcription was used to identify up- and down-regulated genes. We then sequenced rat CBP. Of the IL-1-upregulated genes, CBP and adenine nucleotide translocator-1 (ANT-1) were investigated in vivo. In these experiments, IL-1 was given to rats intraventricularly and sacrificed 2 h later; both CBP and ANT-1 transcripts were upregulated in the cerebral cortex and hypothalamus. We conclude that rat CBP shares many of the functional domains as human and murine CBP and that IL-1 upregulates genes previously associated with learning and sleep.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular Brain Research - Volume 137, Issues 1–2, 13 June 2005, Pages 213-222
نویسندگان
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