کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
9415696 1614321 2005 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Angiotensin II receptors are upregulated by estradiol and progesterone in the locus coeruleus, median preoptic nucleus and subfornical organ of ovariectomized rats
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
Angiotensin II receptors are upregulated by estradiol and progesterone in the locus coeruleus, median preoptic nucleus and subfornical organ of ovariectomized rats
چکیده انگلیسی
Angiotensin II (Ang II) receptors in specific brain areas and in the anterior pituitary are controlled by reproductive hormones. Since Ang II also plays a role in controlling reproductive functions, such as luteinizing hormone and prolactin secretion, the objective of the present study was to evaluate the regulation of Ang II receptors by estradiol (E2) and progesterone (P) in areas of the brain involved in homeostatic and reproductive functions, such as the locus coeruleus (LC), median preoptic nucleus (MnPO) and subfornical organ (SFO). Adult female rats were ovariectomized under anesthesia and divided into 2 groups after 2 weeks: OVX plus E2/P replacement (OVXE2P) and OVX plus oil vehicle (OVX). E2 was injected for 3 consecutive days followed by an injection of P on the 4th day. Animals were killed by decapitation and the brains were removed and frozen. Consecutive coronal brain sections were cut in a cryostat and Ang II receptors were quantified by autoradiography in the MnPO, LC and SFO. Treatment of OVX rats with E2 and P induced a significant increase in the Ang II receptor binding (fmol/mg protein) in the MnPO (OVX: 4.48 ± 0.58 and OVXE2P: 9.89 ± 1.65), LC (OVX: 2.72 ± 0.37 and OVXE2P: 8.03 ± 0.9) and SFO (OVX: 5.45 ± 0.66 and OVXE2P: 10.73 ± 1.79) compared to OVX animals treated with the vehicle, P < 0.05. In conclusion, these results show that Ang II receptors are upregulated by E2 and P in the LC, MnPO and SFO of ovariectomized rats.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Brain Research - Volume 1065, Issues 1–2, 14 December 2005, Pages 47-52
نویسندگان
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