کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9415887 | 1292948 | 2005 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Memantine inhibits ethanol-induced NMDA receptor up-regulation in rat hippocampal neurons
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Memantine inhibits ethanol-induced NMDA receptor up-regulation in rat hippocampal neurons Memantine inhibits ethanol-induced NMDA receptor up-regulation in rat hippocampal neurons](/preview/png/9415887.png)
چکیده انگلیسی
The present study examined the effect of memantine, an uncompetitive NMDA receptor antagonist, on ethanol-induced NMDA receptor up-regulation. Primary glutamatergic rat hippocampal neurons were exposed to ethanol and memantine for 5 days. The ethanol-sensitive NMDA receptor subunits NR1, NR2A and NR2B were quantified by Western immunoblot analysis. Exposure to ethanol (50 mM) caused an increase in the levels of NR1 (137 ± 11% of untreated control, P = 0.009), NR2A (128 ± 14%, P = 0.022) and NR2B (136 ± 19%, P = 0.012). Coincubation with memantine (10 μM) completely blocked the ethanol-induced up-regulation of NR1 (102 ± 4%), NR2A (95 ± 7%) and NR2B (105 ± 13%). No effect of memantine on NR subunit expression was observable, except for NR2A, where a decrease (79 ± 6%, P = 0.034) was noted. Neither ethanol nor memantine alone or in combination were toxic in the concentrations tested. These results may provide a molecular explanation for beneficial effects of memantine on ethanol-induced glutamatergic hyperexcitability reflected in the ethanol withdrawal syndrome and on the development of ethanol dependence.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Brain Research - Volume 1052, Issue 2, 9 August 2005, Pages 156-162
Journal: Brain Research - Volume 1052, Issue 2, 9 August 2005, Pages 156-162
نویسندگان
Juan Manuel Maler, Hermann Esselmann, Jens Wiltfang, Nikolaus Kunz, Piotr Lewczuk, Udo Reulbach, Stefan Bleich, Eckart Rüther, Johannes Kornhuber,