کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9415941 | 1614325 | 2005 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Strain differences in myenteric neuron number and CCK1 receptor mRNA expression may account for differences in CCK induced c-Fos activation
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کلمات کلیدی
FOSNTSOLETFSprague–Dawley - اسپراگ داولیMyenteric plexus - التهاب مزانترImmunohistochemistry - ایمونوهیستوشیمیLong–Evans - بلند اوانزEndocrine and autonomic regulation - تنظیم غدد درون ریز و اتونومیکLETO - سالarea postrema - طبقه آخرdorsal vagal complex - مجتمع وگال پشتیNeuroendocrine regulation - مقررات نوروآندوکرینcholecystokinin - کولهسیستوکینینCCK1 receptor - گیرنده CCK1
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Strain differences in myenteric neuron number and CCK1 receptor mRNA expression may account for differences in CCK induced c-Fos activation Strain differences in myenteric neuron number and CCK1 receptor mRNA expression may account for differences in CCK induced c-Fos activation](/preview/png/9415941.png)
چکیده انگلیسی
We utilized a diaminobenzidine reaction enhanced with nickel to compare dorsal vagal complex (DVC) and myenteric neuronal Fos-Like immunoreactivity (Fos-LI), in response to sulfated cholecystokinin-8 (CCK-8) (5, 10, 20, 40 μg/kg), among Sprague-Dawley (SD), Standard Long-Evans (SLE), Otsuka Long-Evans Tokushima Fatty (OLETF), and Long-Evans Tokushima Otsuka (LETO) rats. All rat strains but OLETF expressed Fos-LI in response to CCK-8. In addition, SD rats expressed more Fos-LI in the area postrema and myenteric neurons than SLE and LETO rats. To investigate the basis for these differences, we utilized cuprolinic blue staining, which stains neuronal cell bodies, to quantify the number of myenteric neurons, and a reverse transcriptase chain polymerase reaction to measure the gene expression of CCK1 receptor in the gut. We found that SD rats have significantly more duodenal myenteric neurons than the other strains. In addition, this strain expressed significantly higher levels of the CCK1 gene in both the duodenum and jejunum than the other strains. In conclusion, SD rats may express more myenteric Fos-LI in response to CCK due to increased numbers of myenteric neurons or more intestinal CCK1 receptors than the other strains of rats.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Brain Research - Volume 1058, Issues 1â2, 5 October 2005, Pages 109-119
Journal: Brain Research - Volume 1058, Issues 1â2, 5 October 2005, Pages 109-119
نویسندگان
Stephen Gulley, Sanjay K. Sharma, Mahmoud Mansour, Cherese N. Sullivan, Timothy H. Moran, Ayman I. Sayegh,