کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9416263 | 1292963 | 2005 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Iptakalim hydrochloride protects cells against neurotoxin-induced glutamate transporter dysfunction in in vitro and in vivo models
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
Parkinson's disease - بیماری پارکینسونUptake and transporters - جذب و حمل و نقلglutamate uptake - جذب گلوتاماتGlutamate transporter - حمل و نقل گلوتاماتExcitable membranes and synaptic transmission - غشاء جذاب و انتقال سیناپسیNeuroprotection - محافظت نورونی یا محافظت از عصبATP-sensitive potassium channel - کانال پتاسیم حساس به ATP
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
Iptakalim hydrochloride (Ipt), a novel antihypertensive drug, exhibits KATP channel activation. Here, we report that Ipt remarkably protects cells against neurotoxin-induced glutamate transporter dysfunction in in vitro and in vivo models. Chronic exposure of cultured PC12 cells to neurotoxins, such as 6-OHDA, MPP+, or rotenone, decreased overall [3H]-glutamate uptake in a concentration-dependent manner. Pre-treatment using 10 μM Ipt significantly protected cells against neurotoxin-induced glutamate uptake diminishment, and this protection was abolished by the KATP channel blocker glibenclamide (20 μM), suggesting that the protective mechanisms may involve the opening of KATP channels. In 6-OHDA-treated rats (as an in vivo Parkinson's disease model), [3H]-glutamate uptake was significantly lower in synaptosomes isolated from the striatum and cerebral cortex, but not the hippocampus. Pre-conditioning using 10, 50, and 100 μM Ipt significantly restored glutamate uptake impairment and these protections were abolished by blockade of KATP channels. It is concluded that Ipt exhibits substantial protection of cells against neurotoxicity in in vitro and in vivo models. The cellular mechanisms of this protective effect may involve the opening of KATP channels. Collectively, Ipt may serve as a novel and effective drug for PD therapy.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Brain Research - Volume 1049, Issue 1, 5 July 2005, Pages 80-88
Journal: Brain Research - Volume 1049, Issue 1, 5 July 2005, Pages 80-88
نویسندگان
Yan-Ling Yang, Chang-Hong Meng, Jian-Hua Ding, Hai-Rong He, Kevin Ellsworth, Jie Wu, Gang Hu,