کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9416727 | 1292981 | 2005 | 17 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Electrical stimulation of the cochlear nerve in rats: analysis of c-Fos expression in auditory brainstem nuclei
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کلمات کلیدی
DCNMTBECICILLDCICc-fosVCNVLLVTBLSODABCICMSOPBSTLTBDll - DLLphosphate buffer - بافر فسفاتtrapezoid body - بدن ترسناکElectrical stimulation - تحریک الکتریکیlateral superior olive - زیتون برتر جانبیmedial superior olive - زیتون عالیSOC - سیستم روی یک تراشهSensory Systems - سیستم های حسیAuditory systems: central anatomy - سیستم های شنوایی: آناتومی مرکزیCochlear nerve - عصب کچلرexternal cortex of the inferior colliculus - قشر خارجی کولیکولوس پایینdorsal cortex of the inferior colliculus - قشر پشتی کولیکولوس پایینLateral lemniscus - لننوسک جانبیsuperior olivary complex - مجتمع عالی ایلواریventral nucleus of the lateral lemniscus - هسته بطنی لنسیسک جانبیlateral nucleus of the trapezoid body - هسته جانبی بدن بدنه استventral cochlear nucleus - هسته حلقوی شکمیventral nucleus of the trapezoid body - هسته شکمی از بدن استپزوئیدmedial nucleus of the trapezoid body - هسته مرکزی بدن تراپزیCentral Nucleus of the Inferior Colliculus - هسته مرکزی کولیکولوس پایینdorsal nucleus of the lateral lemniscus - هسته پشتی لنسیسک جانبیdorsal cochlear nucleus - هسته کچلی پشتیImmediate early genes - ژنهای سریع و سریعInferior colliculus - کولیکولوس پایین
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
We investigated functional activation of central auditory brainstem nuclei in response to direct electrical stimulation of the cochlear nerve using c-Fos immunoreactivity as a marker for functional mapping. The cochlear nerve was stimulated in the cerebellopontine angle of Lewis rats applying biphasic electrical pulses (120-250 μA, 5 Hz) for 30 min. In a control group, bilateral cochlectomy was performed in order to assess the basal expression of c-Fos in the auditory brainstem nuclei. The completeness of cochlear ablations and the response of auditory brainstem nuclei to electrical stimulation were electrophysiologically verified. C-Fos immunohistochemistry was performed using the free floating method. In anaesthetized animals with unilateral electrical stimulation of the cochlear nerve, increased expression of c-Fos was detected in the ipsilateral ventral cochlear nucleus (VCN), in the dorsal cochlear nucleus bilaterally (DCN), in the ipsilateral lateral superior olive (LSO) and in the contralateral inferior colliculus (IC). A bilateral slight increase of c-Fos expression in all subdivisions of the lateral lemniscus (LL) did not reach statistical significance. Contralateral inhibition of the nuclei of the trapezoid body (TB) was observed. Our data show that unilateral electrical stimulation of the cochlear nerve leads to increased expression of c-Fos in most auditory brainstem nuclei, similar to monaural auditory stimulation. They also confirm previous studies suggesting inhibitory connections between the cochlear nuclei. C-Fos immunoreactivity mapping is an efficient tool to detect functional changes following direct electrical stimulation of the cochlear nerve on the cellular level. This could be particularly helpful in studies of differential activation of the central auditory system by experimental cochlear and brainstem implants.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Brain Research - Volume 1031, Issue 1, 7 January 2005, Pages 39-55
Journal: Brain Research - Volume 1031, Issue 1, 7 January 2005, Pages 39-55
نویسندگان
Makoto Nakamura, Steffen K. Rosahl, Eyad Alkahlout, Gerhard F. Walter, Madjid M. Samii,