Sensitivity of surface EMG-based conduction velocity estimates to local tissue in-homogeneities - influence of the number of channels and inter-channel distance

The aim of this simulation study was to investigate the influence of local tissue in-homogeneities on the estimates of muscle fiber conduction velocity (CV) from surface EMG signals. A recently developed analytical surface EMG model was used to generate simulated surface EMG signals from a planar layered volume conductor, comprised of the muscle tissue and fat layer, with spheres (1 mm radius) in the fat layer of conductivity different from the surrounding tissue. CV was estimated with a maximum likelihood multi-channel approach, varying the number of channels and the inter-channel distance used for the estimate. The action potentials detected along the muscle fiber direction changed shape due to the presence of the in-homogeneities, thus affecting CV estimates. CV estimates were influenced by the location of the in-homogeneities with respect to the fiber and detection electrodes. The maximum percent variation of CV estimates due to the presence of in-homogeneities decreased with increasing number of channels and inter-channel distance: 19.6% (2 channels), 12.1% (3 channels), 6.4% (4 channels), for 5 mm inter-channel distance, and 12.0% (2 channels), 5.2% (3 channels), 2.4% (4 channels), for 10 mm inter-channel distance (for double differential detection). The results were in agreement and explained previous experimental findings. It was concluded that multi-channel methods for CV estimation significantly reduce the sensitivity of CV estimates to tissue in-homogeneities.