کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9424254 | 1295256 | 2005 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
RNA amplification of bromodeoxyuridine labeled newborn neurons in the monkey hippocampus
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: RNA amplification of bromodeoxyuridine labeled newborn neurons in the monkey hippocampus RNA amplification of bromodeoxyuridine labeled newborn neurons in the monkey hippocampus](/preview/png/9424254.png)
چکیده انگلیسی
Neurogenesis has been demonstrated in the adult mammalian hippocampus by the immunohistochemical identification of cells co-labeled with the neuronal marker NeuN and bromodeoxyuridine (BrdU), a marker for DNA synthesis. Whether these newly born neurons exhibit a genetic signature similar to that of existing hippocampal cells remains unknown. Recent advances in single cell RNA amplification techniques provide a unique method for profiling the mRNA complement of cells developed during adult neurogenesis. Standard protocols for identifying BrdU-positive cells requires an acid denaturation step that may preclude the amplification of cellular RNA for expression analysis. We first tested whether the BrdU reaction product was visible in monkey hippocampal tissue following treatment with dilutions of HCl (2-0.2Â M) or citric acid (1.0-0.1Â M). BrdU-labeled cells were visible only in tissue sections treated with 2Â M HCl. RNA amplification was not compromised in cells dual-labeled for BrdU and NeuN using the 2Â M HCl acid denaturation step. These cells express mRNAs encoding a wide variety of functional protein subclasses including glutamate receptors. The present study demonstrates for the first time that BrdU immunohistochemisty is compatable with gene array technology in the primate hippocampus to evaluate subclasses of genes in newborn neurons.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Neuroscience Methods - Volume 144, Issue 2, 15 June 2005, Pages 197-201
Journal: Journal of Neuroscience Methods - Volume 144, Issue 2, 15 June 2005, Pages 197-201
نویسندگان
Scott E. Counts, Er-Yun Chen, Stephen D. Ginsberg, Jeffrey H. Kordower, Elliott J. Mufson,