کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9424260 | 1295256 | 2005 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Extracellular trypsin increases ASIC1a selectivity for monovalent versus divalent cations
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
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چکیده انگلیسی
Sustained proton activation of native ASIC channels in primary sensory neurons or HEK293 cells leads to a reduction in the peak amplitude of transient inward currents and the progressive development of a persistent component, which hinders titration experiments in pharmacological studies. Here we report that extracellular trypsin applied for 5 min at 10-45 μg/ml and/or a short exposure to high Ca2+ (75 mM for less than 1 min) alleviate the persistent component, improving reproducibility of acid-elicited transients. Selectivity measurements performed in current clamp mode, in essentially bi-ionic conditions, prove that these two treatments decrease hASIC1a permeability for divalent but not for monovalent cations, producing a significant change in PNa/PCa from 8.2 ± 2.1 (mean ± S.D.) to 26.0 ± 7.8 (trypsin) or 24.5 ± 11.1 (high Ca2+). The slope conductance of the unit inward Ca2+ transient was also lowered from 5.7 to 2.7 pS after trypsin.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Neuroscience Methods - Volume 144, Issue 2, 15 June 2005, Pages 241-248
Journal: Journal of Neuroscience Methods - Volume 144, Issue 2, 15 June 2005, Pages 241-248
نویسندگان
Emil Neaga, Bogdan Amuzescu, Cristina Dinu, Beatrice Macri, Florentina Pena, Maria-Luiza Flonta,