کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9425771 | 1295890 | 2005 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Inhibition of the calcium- and voltage-dependent big conductance potassium channel ameliorates cisplatin-induced apoptosis in spiral ligament fibrocytes of the cochlea
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
PBSα-MEMAnnexin V-FITCα-minimum essential medium - α حداقل محیط ضروریTetraethylammonium - تترا اتیل آمونیومApoptosis - خزان یاختهایOtotoxicity - سمیت زایی برای گوش، اتوتوکسیسیتیcisplatin - سیس پلاتینPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریendocochlear potential - پتانسیل آندوکولارTEA - چایBK channel - کانال BKK channel - کانال KGerbil - گربیلInner ear - گوش داخلی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The role of calcium- and voltage-dependent big conductance potassium channels in regulating apoptosis was investigated in cultured type I spiral ligament fibrocytes. Incubation of type I spiral ligament fibrocytes derived from gerbil cochlea with cisplatin induced dose- and time-dependent apoptosis as demonstrated by annexin V conjugated to fluorescein isothiocyanate/prodidium iodide assays. The average voltage activation threshold of whole cell current was sharply shifted to â40 mV in the cisplatin-treated cells as compared with a value of 40 mV in control cells. The average whole-cell current of cisplatin-treated cells induced by a depolarization voltage step from â80 to â10 mV was increased significantly to 1.2±0.4 nA as compared with 0.08±0.1 nA in control cells. Coincubation with tetraethylammonium and cisplatin retained the whole cell current in the normal range (0.12±0.2 nA). The increment of cisplatin-induced whole-cell current was inhibited (97±5%) by a specific calcium- and voltage-dependent big conductance potassium channel blocker iberiotoxin. Consistent with this, co-incubation with tetraethylammonium significantly attenuated cisplatin-induced apoptosis in type I spiral ligament fibrocytes by more than 50%. We conclude that the activation of BK channels is an early event associated with cisplatin-induced apoptosis in type I spiral ligament fibrocytes. These findings also point to the calcium- and voltage-dependent big conductance potassium channels as a potential pharmacological target for manipulating cisplatin ototoxicity.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 135, Issue 1, 2005, Pages 263-271
Journal: Neuroscience - Volume 135, Issue 1, 2005, Pages 263-271
نویسندگان
F. Liang, B.A. Schulte, C. Qu, W. Hu, Z. Shen,