کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9426233 | 1295913 | 2005 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Corticosterone and dexamethasone potentiate cytotoxicity associated with oxygen-glucose deprivation in organotypic cerebellar slice cultures
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کلمات کلیدی
DMEMRU486N-methyl-d-aspartateNMDAOGDHBSSU.S.DMSO - DMSODulbecco’s modified Eagle medium - Modified Eagle اصلاح شده DulbeccoUnited States - ایالات متحده آمریکاTrolox - ترولکسNeuronal damage - خسارت عصبیDimethyl sulfoxide - دیمتیل سولفواکسیدheat-inactivated horse serum - سرم اسب سرما گرانقیمتHIHS - سلامStroke - سکته مغزیOxygen-glucose deprivation - محرومیت گلوکز اکسیژنHanks’ balanced salt solution - محلول نمک متعادل هانکسGlucocorticoid - گلوکوکورتیکوئیدهاGlucocorticoids - گلوکوکورتیکوئیدهاglucocorticoid receptor - گیرنده گلوکوکورتیکوئید
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Many patients display elevated levels of serum cortisol following acute ischemic stroke. Given that glucocorticoids may potentiate some forms of insult, these studies examined the effects of corticosterone or dexamethasone exposure on cytotoxicity following oxygen-glucose deprivation in the cerebellum, a brain region susceptible to stroke. In organotypic cerebellar slice cultures prepared from neonatal rat pups, 90-min of oxygen-glucose deprivation at 15 days in vitro resulted in significant cytotoxicity at 24-, 48-, and 72-h post-oxygen-glucose deprivation, as measured by uptake of propidium iodide. Exposure of cultures following oxygen-glucose deprivation to the antioxidant trolox (500μM), but not to the glucocorticoid receptor antagonist RU486 (10μM), completely blocked oxygen-glucose deprivation-induced cytotoxicity. Corticosterone (1μM) or dexamethasone (10μM) exposure alone did not significantly increase propidium iodide uptake above levels observed in control cultures. However, corticosterone or dexamethasone exposure after oxygen-glucose deprivation potentiated oxygen-glucose deprivation-mediated propidium iodide uptake at each time point. Trolox, as well as RU486, co-exposure of cultures to corticosterone or dexamethasone after oxygen-glucose deprivation abolished all cytotoxicity. In conclusion, these data demonstrated that glucocorticoid exposure modulated oxygen-glucose deprivation-mediated propidium iodide uptake, which likely involved glucocorticoid receptor activation and pro-oxidant effects.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 136, Issue 1, 2005, Pages 259-267
Journal: Neuroscience - Volume 136, Issue 1, 2005, Pages 259-267
نویسندگان
P.J. Mulholland, T.D. Stepanyan, R.L. Self, A.K. Hensley, B.R. Harris, A. Kowalski, J.M. Littleton, M.A. Prendergast,