کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9429480 | 1297045 | 2005 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A tetracycline-regulatable adeno-associated virus vector for double-gene transfer
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: A tetracycline-regulatable adeno-associated virus vector for double-gene transfer A tetracycline-regulatable adeno-associated virus vector for double-gene transfer](/preview/png/9429480.png)
چکیده انگلیسی
An increasing demand for polycistronic vectors that express multiple genes simultaneously has arisen in recent years to obtain an efficient gene therapy. Armed with the knowledge that the expression of transgene in mammalian cells often requires tight control, we constructed in this study a tetracycline-regulated double-gene adeno-associated virus (AAV) vector carrying green and red fluorescent protein genes and expressed it in PC12 cells. When detected by fluorescence microscope and fluorescence-activated cell sorting, gene expression was induced by 44-66-fold and could be reversibly controlled by doxycycline. This double-gene AAV vector may be useful for regulated expression of two genes or a marker to monitor transgene expression.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience Letters - Volume 378, Issue 2, 18 April 2005, Pages 106-110
Journal: Neuroscience Letters - Volume 378, Issue 2, 18 April 2005, Pages 106-110
نویسندگان
Jian-jun Wang, Dong-bin Niu, Ting Zhang, Kun Wang, Bing Xue, Xiao-min Wang,