کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9436487 | 1615691 | 2005 | 13 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Differential distribution of β- and γ-actin in guinea-pig cochlear sensory and supporting cells
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کلمات کلیدی
tetramethyl rhodamine isothiocyanateFITCStereociliaTBSTRITCPBSOHCIHC - ایمونوهیستوشیمیTem - این استphosphate buffer - بافر فسفاتTris buffered saline - تریس نمک بافرouter hair cell - سلول بیرونی موInner hair cell - سلول داخلی داخلیHair cells - سلول های مویAuditory system - سیستم شنواییPhosphate buffered saline - فسفات بافر شورfluorescein isothiocyanate - فلوئورسین ایسوتیوسیاناتactin filaments - فیبرهای اکتینTransmission electron microscope - میکروسکوپ الکترونی انتقالmap - نقشهmicrotubule associated protein - پروتئین مرتبط با میکروتوبول
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
سیستم های حسی
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چکیده انگلیسی
Sensory and supporting cells of the mammalian organ of Corti have cytoskeletons containing β- and γ-actin isoforms which have been described as having differing intracellular distributions in chick cochlear hair cells. Here, we have used post-embedding immunogold labelling for β- and γ-actin to investigate semiquantitatively how they are distributed in the guinea-pig cochlea and to compare different frequency locations. Amounts of β-actin decrease and γ-actin increase in the order, outer pillar cells, inner pillar cells, Deiters' cells and hair cells. There is also more β-actin and less γ-actin in outer pillar cells in higher than lower frequency regions. In hair cells, β-actin is present in the cuticular plate but is more concentrated in the stereocilia, especially in the rootlets and towards the periphery of their shafts; labelling densities for γ-actin differ less between these locations and it is the predominant isoform of the hair-cell lateral wall. Alignments of immunogold particles suggest β-actin and γ-actin form homomeric filaments. These data confirm differential distribution of these actin isoforms in the mammalian cochlea and reveal systematic differences between sensory and supporting cells. Increased expression of β-actin in outer pillar cells towards the cochlear base may contribute to the greater stiffness of this region.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Hearing Research - Volume 207, Issues 1â2, September 2005, Pages 22-34
Journal: Hearing Research - Volume 207, Issues 1â2, September 2005, Pages 22-34
نویسندگان
D.N. Furness, Y. Katori, S. Mahendrasingam, C.M. Hackney,