Biodegradation and detoxification of p-nitrophenol by Rhodococcus wratislaviensis

Biodegradation was evaluated by spectrophotometry, gas chromatography and microbial growth. As the nitrite released during PNP degradation is also an environmental toxic agent it was removed by adding a denitrification step in an anoxic process. Detoxification was evaluated by using a Daphnia magna toxicity test. Toxicity was not detected after the combination of both, an aerobic biodegradation test and denitrification in an anoxic process.