کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9442602 | 1302565 | 2005 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Isolation and characterization of a cDNA encoding a serine proteinase from the root-knot nematode Meloidogyne incognita
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کلمات کلیدی
RT-PCRMeloidogyneRKNEST - استrapid amplification of cDNA ends - تقویت سریع cDNA به پایان می رسدExpressed Sequence Tag - تکرار اظهار نظرreverse transcription and polymerase chain reaction - رونویسی معکوس و واکنش زنجیره ای پلیمرازRace - مسابقهRoot-knot nematode - نماتد ریشه گرهSerine proteinase - پروتئین سینNematode - کرمهای لولهایcDNA cloning - کلونینگ cDNA
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
انگل شناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Isolation and characterization of a cDNA encoding a serine proteinase from the root-knot nematode Meloidogyne incognita Isolation and characterization of a cDNA encoding a serine proteinase from the root-knot nematode Meloidogyne incognita](/preview/png/9442602.png)
چکیده انگلیسی
This report describes the first serine proteinase gene isolated from the sedentary nematode Meloidogyne incognita. Using degenerate primers, a 1372Â bp cDNA encoding a chymotrypsin-like serine proteinase (Mi-ser1) was amplified from total RNA of adult females by RT-PCR and 5â² and 3â² rapid amplification of cDNA ends. The deduced amino acid sequence of Mi-ser1 encoded a putative signal peptide and a prodomain of 22 and 33 amino acids, respectively, and a mature proteinase of 341 amino acids with a predicted molecular mass of 37,680Â Da. Sequence identity with the top serine proteinases matches from the databases ranged from 23 to 27%, including sequences from insects, mammals, and other nematodes. Southern blot analysis suggested that Mi-ser1 is encoded by a single or few gene copies. The pattern of developmental expression analyzed by Northern blot and RT-PCR indicated that Mi-ser1 was transcribed mainly in females. The domain architecture composed of a single chymotrypsin-like catalytic domain and the detection of a putative signal peptide suggested a digestive role for Mi-ser1.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Parasitology - Volume 110, Issue 2, June 2005, Pages 123-133
Journal: Experimental Parasitology - Volume 110, Issue 2, June 2005, Pages 123-133
نویسندگان
Rodrigo da Rocha Fragoso, João Aguiar Nogueira Batista, Osmundo Brilhante Oliveira Neto, Maria Fátima Grossi de Sá,