کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9442663 | 1302572 | 2005 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Lung-migrating digenean parasites: in vitro influence on nitric oxide production from normal rat pulmonary macrophages
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
PBSRT-PCRiNOSDigeneaSchistosoma bovisinducible nitric synthasel-NAME - L-NAMELung - ریهMacrophage - ماکروفاژ Phosphate-buffered saline - محلول نمک فسفات با خاصیت بافریnitro-l-arginine methyl ester - نیترو-ال-آرژینین متیل استرNitric oxide - نیتریک اکسیدreverse transcription-polymerase chain reaction - واکنش زنجیره ای رونویسی-پلیمراز معکوس
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
انگل شناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Lung-migrating digenean parasites: in vitro influence on nitric oxide production from normal rat pulmonary macrophages Lung-migrating digenean parasites: in vitro influence on nitric oxide production from normal rat pulmonary macrophages](/preview/png/9442663.png)
چکیده انگلیسی
Nitric oxide (NO) is one of the most versatile players in the immune system. Most parasites induce inflammation in the host associated with NO production. Here, we compare the in vitro effect of Schistosoma bovis somatic (SbS) and excretory-secretory (SbES) antigens, and excretory-secretory Paragonimus mexicanus adult worm (PmES) molecules on rat alveolar macrophages NO production measured by the Griess method and by RT-PCR. Additionally, we address the divergence of the NO stimulatory/inhibitory effects of these two parasites. Polymyxin B was used to assess possible LPS contamination. In vitro incubation of rat alveolar macrophages with PmES (⩾10 μg/ml) and SbS (⩾50 μg/ml), but not with SbES extracts, resulted in NO production and an increase in iNOS cell mRNA. This production was specific and inhibited by L-NAME and l-canavanine. Different effects were observed when cells were incubated with P. mexicanus and S. bovis antigens.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Parasitology - Volume 109, Issue 3, March 2005, Pages 171-175
Journal: Experimental Parasitology - Volume 109, Issue 3, March 2005, Pages 171-175
نویسندگان
M. Amparo Andrade, Mar Siles-Lucas, Julio López-Abán, José Luis Pérez-Arellano, Ivan D. Vélez, Antonio Muro,