کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9442665 | 1302572 | 2005 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Giardia lamblia: stable expression of green fluorescent protein mediated by giardiavirus
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موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
انگل شناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Giardia lamblia: stable expression of green fluorescent protein mediated by giardiavirus Giardia lamblia: stable expression of green fluorescent protein mediated by giardiavirus](/preview/png/9442665.png)
چکیده انگلیسی
Giardia lamblia, an early diverging eukaryote that infects several species including humans and a major agent of water-borne diarrhea throughout the world, can be infected with a double-stranded RNA virus, giardiavirus (GLV). A chimeric GLV cDNA and green fluorescent protein (GFP) according to the cis-acting signals of the GLV genome required for expression of foreign gene was constructed and its in vitro transcript was electroporated into GLV-infected G. lamblia trophozoites, GFP was expressed transiently. pGDH5/NEO/GLV was constructed by combining the neomycin resistance cassette in which the neomycin phosphotransferase gene was flanked by Giardia glutamate dehydrogenase (GDH) uncoding regions and the transcription cassette in which the chimera of GLV cDNA and GFP was located downstream from GDH gene promoter on a single plasmid. This plasmid was electroporated into G. lamblia and the transfectants persistently expressed GFP under G418 selection. This stable transfection system should provide a valuable tool for genetic study of G. lamblia.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Parasitology - Volume 109, Issue 3, March 2005, Pages 181-187
Journal: Experimental Parasitology - Volume 109, Issue 3, March 2005, Pages 181-187
نویسندگان
Quan Liu, Xichen Zhang, Jianhua Li, Jigang Ying, Lifeng Chen, YunJun Zhao, Feng Wei, Tao Wu,