کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9442844 | 1302594 | 2005 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Echinococcus granulosus: Lethal effect of low voltage direct electric current on hydatid cyst protoscoleces
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
انگل شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
We have studied a small scale method for killing hydatid cyst protoscoleces using low voltage direct electric current. After collecting hydatid cysts from infected organs of slaughtered animals, protoscoleces were cultured in four different media: hydatid cyst fluid, RPMI, normal saline, and Tris buffer, respectively. Protoscoleces from each of the above media were then transferred to an electrolysis device through which different electric current densities were applied. For measuring the survival rate of protoscoleces, flame cell movement and eosin staining was used. The results show that the survival rate of protoscoleces in hydatid fluid was dependent on the electric current density and the time of the applied current. Current densities of 62.5Â mA/cm2 (11Â V), 53.71Â mA/cm2 (10Â V), and 18.18Â mA/cm2 (5Â V) after 1, 2, and 3Â min, respectively, killed all the parasites in the hydatid fluid. However, a current density of 7Â mA/cm2 (9Â V) in RPMI medium after 3Â min was most effective.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Parasitology - Volume 109, Issue 4, April 2005, Pages 237-240
Journal: Experimental Parasitology - Volume 109, Issue 4, April 2005, Pages 237-240
نویسندگان
A. Dalimi, R. Ghasemikhah, B. Hashemi Malayeri,